Couverture / Jaquette

With extensive coverage of synthesis techniques and applications, this text describes chemical biology techniques which have gained significant impetus during the last five years. It focuses on the methods for obtaining modified and native nucleic acids, and their biological applications. Topics covered include:* chemical synthesis of modified RNA* expansion of the genetic alphabet in nucleic acids by creating new base pairs* chemical biology of DNA replication: probing DNA polymerase selectivity mechanisms with modified nucleotides* nucleic-acid-templated chemistry* chemical biology of peptide nucleic acids (PNA)* the interactions of small molecules with DNA and RNA* the architectural modules of folded RNAs* genesis and biological applications of locked nucleic acid (LNA)* small non-coding RNA in bacteria* microRNA-guided gene silencing* nucleic acids based therapies* innate immune recognition of nucleic acid* light-responsive nucleic acids for the spatiotemporal control of biological processes* DNA methylation* frameworks for programming RNA devices* RNA as a catalyst: The Diels-Alderase-Ribozyme* evolving an understanding of RNA function by in vitro approaches* the chemical biology of aptamers: synthesis and applications* nucleic acids as detection tools* bacterial riboswitch discovery and analysisThe Chemical Biology of Nucleic Acids is an essential compendium of the synthesis of nucleic acids and their biological applications for bioorganic chemists, chemical biologists, medicinal chemists, cell biologists, and molecular biologists.

Note biographique

Günter Mayer, Assistant Professor, LIMES Prog. Unit Chemical Biology and Medicinal Chemistry, Kekulé-Institute for Organic Chemistry and Biochemistry, University of Bonn, Germany
Professor Mayer's research involves molecular diversity applied to elucidate the conformation, function and identity of biomolecules. He is a co-founder of NascaCell Technologies AG, a leading service provider of innovative biomolecules, where he headed the combinatorial biotechnology department. He has published papers in leading journals such as Nature, Angewandte Chemie and Journal of the American Chemical Society.

Table des matières

Foreword vii

Preface ix

List of Contributors xi

1 Chemical Synthesis of Modified RNA 1
Claudia Höbartner and Falk Wachowius

2 Expansion of the Genetic Alphabet in Nucleic Acids by Creating New Base Pairs 39
Ichiro Hirao and Michiko Kimoto

3 Chemical Biology of DNA Replication: Probing DNA Polymerase Selectivity Mechanisms with Modified Nucleotides 63
Andreas Marx

4 Nucleic Acid-templated Chemistry 73
Michael Oberhuber

5 Chemical Biology of Peptide Nucleic Acids (PNAs) 103
Peter E. Nielsen

6 The Interactions of Small Molecules with DNA and RNA 115
Yun Xie, Victor K. Tam and Yitzhak Tor

7 The Architectural Motifs of Folded RNAs 141
Valérie Fritsch and Eric Westhof

8 Genesis and Biological Applications of Locked Nucleic Acids (LNAs) 175
Harleen Kaur and Souvik Maiti

9 Small Non-coding RNA in Bacteria 199
Sabine Brantl

10 MicroRNA-guided Gene Silencing 223
Gunter Meister

11 Nucleic Acid-based Therapies 233
Britta Hoehn and John J. Rossi

12 Innate Immune Recognition of Nucleic Acids 261
Stefan Bauer

13 Light-responsive Nucleic Acids for the Spatiotemporal Control of Biological Processes 279
Alexander Heckel and Günter Mayer

14 DNA Methylation 307
Albert Jeltsch and Renata Z. Jurkowska

15 Frameworks for Programming RNA Devices 323
Maung Nyan Win, Joe C. Liang and Christina D. Smolke

16 RNA as a Catalyst: The Diels-Alderase Ribozyme 339
Andres Jäschke

17 Evolving an Understanding of RNA Function by In Vitro Approaches 355
Qing Wang and Peter J. Unrau

18 The Chemical Biology of Aptamers: Synthesis and Applications 377
Günter Mayer and Bernhard Wulffen

19 Nucleic Acids as Detection Tools 401
Jeffrey C.F. Lam, Sergio Aguirre and Yingfu li

20 Bacterial Riboswitch Discovery and Analysis 433
Tyler D. Ames and Ronald R. Breaker

Index 455

Détails